![]() In the presence of target, the hybridization of the probe region with the target is energetically moreįavorable than preserving the duplex in the stem, which leads to the separation of the fluorophore/quencher pair and a significant Sequence complementary to the mRNA target (18–26 nucleotides ), and to the double-stranded region (five G/C-rich base In this study, we use the terms “probe” and “stem” to refer to the loop or single-stranded region of the hairpin with a Molecular beacons are oligonucleotides labeled with a fluorophore at the 5′ end and a quencher at the 3′ end that are designed We use the molecular beacon technology for live cell imaging of mRNA transport and localization in the fruit fly egg chamber Previous Section Next Section INTRODUCTIONĬurrently, there are a few methods that are used to image RNA in live tissues and cells, and each has its advantages and disadvantages Here, we show that osk1236 outperformed osk2216 in live cell imaging experiments. We previously demonstrated osk2216 to be efficient in detecting oskar mRNA in in vivo experiments. To demonstrate its applicability, we synthesized an oskar mRNA-specific molecular beacon (osk1236), which is selected by PinMol to target a more accessible region than a manually designed oskar-specific molecular beacon (osk2216). PinMol takes into account the accessibility of the targeted regions, as well as the inter- and intramolecular interactions of each Of the target RNA, predicted via energy minimization approaches. Here, we report a Python program ( PinMol) that designs molecular beacons best suited for live cell imaging by using structural information from secondary structures They are used for the visualization of endogenous mRNAs in live cells. Which fluoresce only when bound to their target RNA. I will, of course, still continue my art director and graphic design endeavors as well.Molecular beacons are nucleic acid oligomers labeled with a fluorophore and a quencher that fold in a hairpin-shaped structure, It's a side of my career that I've pursued for most of my life. ![]() Here you can view (and purchase if you like) recent watercolors I've painted. I've created a second website that deals solely with the fine art painter/watercolor side of my career. New York City & New Port Richey, Florida (Gulf of Mexica coastline) both on the creative side as well as the budget side! You'll be extremely happy with the results. I'm always available to discuss any upcoming project that you have on your calendar. So whether you’re big or small, or any size in between, feel free to contact me any time. I also work with advertising agencies and varied design firms helping them on projects when their work load becomes too great. And I’ve designed the menu and logo for one of my favorite local restaurants (with a guaranteed great table every time I go there!). I've created major advertising campaigns for large multinational companies. I work with a wide variety of clients, both large and small. ![]() Pharmaceutical Sales Aids & related materials Stationary (Letterheads, Envelopes, Business Cards) Print Ads (Magazine & Newspaper Advertising) My diverse portfolio includes memorable, innovative solutions for: Because my responsibility as an art director is not only visual, but also includes the development of the overall advertising concept, many of the samples shown in this section showcase concepts that contain both visuals and headlines that were conceived by me alone. On the art director side, the ads button will be most helpful in evaluating my work. All done at an affordable cost that won't break the budget. I encourage you to also take a look at the design/logos, web/motion and web/web design sections, which show the diversified range of print and web graphics that I've created. It contains about a third of all the samples on the site. ![]() With over 75 samples shown here, you can get a pretty good idea of the kind of work I do.įor those interested in my graphic design side, the design/print section is the place to go. This website is my art direction and graphic design portfolio. Concurrent with my career as an art director and designer I've also been an Adjunct College Instructor teaching advertising concept and design at two large east coast universities.I pride myself in offering clients the outstanding personal service that a small studio makes possible, while giving them the kind of high quality work matched only by the very best firms in this business. For over 20 years I've worked as an Art Director, Graphic Designer and Web Designer creating award winning projects in some of the world’s largest advertising agencies in New York City.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |